Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 23.6% (w/v) PEG 4000, 0.1 M HEPES, pH 8.0 | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48170 | - |
X-ray crystallography | Staphylococcus aureus |
50000 | - |
SDS-PAGE | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A163U8E7 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose 6 fast flow chromatography, HiPrep 26/10 desalting column chromatography, and S-75 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 2-oxoglutarate | - |
Staphylococcus aureus | 4-hydroxyphenylpyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | S-75 gel filtration | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
Sar2028 | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent | Staphylococcus aureus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus aureus | 2D-PAGE | - |
5.25 |